Anticorps Monoclonal anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F8H3

N° de cat : 67337-1-PBS

Synonymes

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Galerie de données de validation

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  WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  WB analysis of SW480 using 67337-1-Ig (same clone as 67337-1-PBS)

  SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67337-1-Ig (same clone as 67337-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67337-1-Ig (same clone as 67337-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 67337-1-Ig (same clone as 67337-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67337-1-Ig (same clone as 67337-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67337-1-PBS cible SIRP Alpha/CD172a dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRP Alpha/CD172a Protéine recombinante Ag6361
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC033092
• Symbole du gène: SIRP Alpha
• Identification du gène (NCBI): 140885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

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