Anticorps Polyclonal de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Polyclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31485-1-PBS

Synonymes

SIRP Alpha, SIRPA, MFR, Inhibitory receptor SHPS-1, CD172a

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Galerie de données de validation

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  WB analysis using 31485-1-AP (same clone as 31485-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31485-1-PBS in a different storage buffer formulation.

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  WB analysis using 31485-1-AP (same clone as 31485-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31485-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 31485-1-AP (same clone as 31485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (31485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31485-1-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 31485-1-AP (same clone as 31485-1-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 31485-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31485-1-PBS cible SIRP Alpha/CD172a dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 65-100 kDa
• Numéro d’acquisition GenBank: NM_080792
• Symbole du gène: SIRP Alpha
• Identification du gène (NCBI): 140885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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