Anticorps Recombinant de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

240789E1

N° de cat : 98101-1-PBS

Synonymes

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Galerie de données de validation

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  FC experiment of human peripheral blood leukocytes using 98101-1-RR (same clone as 98101-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98101-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98101-1-RR (same clone as 98101-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRPA Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98101-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98101-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98101-1-RR against Human SIRP Alpha/CD172a were performed. The affinity constant is 3.39 nM.

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Informations sur le produit

98101-1-PBS cible SIRP Alpha/CD172a dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 55 kDa
• Numéro d’acquisition GenBank: NM_080792
• Symbole du gène: SIRP Alpha
• Identification du gène (NCBI): 140885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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