Anticorps Recombinant de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

Non conjugué

CloneNo.

241794D9

N° de cat : 98225-1-RR

Synonymes

241794D9, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using 98225-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD11b. Cells were not fixed.

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  FC experiment of mouse bone marrow cells using 98225-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse SIRP alpha/CD172a Rabbit Recombinant Antibody (98225-1-RR, Clone: 241794D9) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98225-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98225-1-RR against Mouse SIRP Alpha/CD172a were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98225-1-RR cible SIRP Alpha/CD172a dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SIRP Alpha/CD172a Protéine recombinante Eg1520
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 56 kDa
• Numéro d’acquisition GenBank: NM_007547.4
• Symbole du gène: Sirpa
• Identification du gène (NCBI): 19261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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