Anticorps Recombinant de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241794D9

N° de cat : CL647-98225

Synonymes

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using CL647-98225

  1x10^6 mouse bone marrow cells were surface stained with FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone: M1/70), and 0.25 ug CoraLite® Plus 647 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL647-98225, Clone: 241794D9) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using CL647-98225

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse SIRP Alpha/CD172a Rabbit RecAb (CL647-98225, Clone: 241794D9) (red) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-98225 cible SIRP Alpha/CD172a dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 56 kDa
• Numéro d’acquisition GenBank: NM_007547.4
• Symbole du gène: Sirpa
• Identification du gène (NCBI): 19261
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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