Anticorps Recombinant de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241794D9

N° de cat : 84430-2-PBS

Synonymes

241794D9, Bit, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a

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Galerie de données de validation

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  WB analysis using 84430-2-RR (same clone as 84430-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

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  WB analysis using 84430-2-RR (same clone as 84430-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01311-4

  Sandwich ELISA standard curve of MP01311-4, Mouse SIRP alpha/CD172a Recombinant Matched Antibody Pair - PBS only. 84430-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1520. 84430-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

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  Sample test of MP01311-4

  The mean SIRP alpha/CD172a concentration was determined to be 1,352.0 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load, 1,425.8 ng/mL in mouse cerebellum tissue extract based on a 2.9 mg/mL extract load, 796.6 ng/mL in RAW 264.7 cell extract based on a 4.2 mg/mL extract load and 4.0 ng/mL in NIH/3T3 cell extract based on a 4.4 mg/mL extract load.

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  Sample test of MP01311-4

  The mean SIRP alpha/CD172a concentration was determined to be 595.2 ng/mL in mouse brain tissue homogenate based on a 2.2 mg/mL extract load.

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  Sample test of MP01311-4

  Serum of six mice was measured. The SIRP alpha/CD172a concentration of detected samples was determined to be 69.0 ng/mL with a range of 59.9-73.9 ng/mL.

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  IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 84430-2-RR (same clone as 84430-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (84430-2-RR, Clone: 241794D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.

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Informations sur le produit

84430-2-PBS cible SIRP Alpha/CD172a dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SIRP Alpha/CD172a Protéine recombinante Eg1520
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 90-140 kDa
• Numéro d’acquisition GenBank: NM_007547.4
• Symbole du gène: Sirpa
• Identification du gène (NCBI): 19261
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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