Anticorps Polyclonal de lapin anti-SIRP Alpha/CD172a

• Phare
• Validé par KD/KO

SIRP Alpha/CD172a Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (2)

N° de cat : 14482-1-AP

Synonymes

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

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Galerie de données de validation

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14482-1-AP

  WB result of SIRP Alpha/CD172a antibody (14482-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRP Alpha/CD172a transfected U-937 cells.

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 14482-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14482-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 14482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (14482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules HL-60, cellules PC-12, cellules U-937, tissu de cervelet de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

14482-1-AP cible SIRP Alpha/CD172a dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRP Alpha/CD172a Protéine recombinante Ag5865
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC033092
• Symbole du gène: SIRP Alpha
• Identification du gène (NCBI): 140885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Publications

Species	Application	Title
mouse	WB	Cell Prolif; Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling; Authors - Qing Tu
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.; Authors - Kejia Zhao
human	WB	J Obstet Gynaecol Res; Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.; Authors - Qi Xie
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manuel (Verified Customer) (03-19-2025)

Works well.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: THP-1

FH

Rebecca (Verified Customer) (05-27-2024)

Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-CM

SIRP Alpha/CD172a Antibody Western Blot validation (1:1000 dilution) in hiPSC-CM (Cat no:14482-1-AP)

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