Anticorps Recombinant de lapin anti-SIRP Alpha/CD172a

SIRP Alpha/CD172a Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240789C6

N° de cat : 83729-5-PBS

Synonymes

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Galerie de données de validation

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  WB analysis of U-937 using 83729-5-RR (same clone as 83729-5-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 83729-5-RR (same clone as 83729-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83729-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

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Informations sur le produit

83729-5-PBS cible SIRP Alpha/CD172a dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: signal-regulatory protein alpha
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: NM_080792
• Symbole du gène: SIRP Alpha
• Identification du gène (NCBI): 140885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

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