Anticorps Multi-Recombinant de lapin anti-SIRT1

SIRT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : RMX00009

Synonymes

EC:2.3.1.-, EC:2.3.1.286, hSIR2, hSIRT1, NAD-dependent protein deacetylase sirtuin-1

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Galerie de données de validation

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  WB analysis using RMX00009

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using RMX00009

  IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.

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  IHC staining of human colon cancer using RMX00009

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

RMX00009 cible SIRT1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Multi-Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Masse moléculaire calculée: 747 aa, 82 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: BC012499
• Symbole du gène: SIRT1
• Identification du gène (NCBI): 23411
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: N/A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein containing one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.

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