Anticorps Polyclonal de lapin anti-SIRT2

• Phare
• Validé par KD/KO

SIRT2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

49

N° de cat : 19655-1-AP

Synonymes

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2, SIRT2-Specific

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Galerie de données de validation

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  WB analysis using 19655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19655-1-AP (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19655-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19655-1-AP (SIRT2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19655-1-AP

  IP result of anti-SIRT2-Specific (IP:19655-1-AP, 4ug; Detection:19655-1-AP 1:800) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human heart using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of rat brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 19655-1-AP (SIRT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IHC staining of human heart using 19655-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19655-1-AP (SIRT2-Specific antibody at dilution of 1:50.

• 

  IF Staining of mouse cortex using 19655-1-AP

  IF results of SIRT2 (19655-1-AP) antibody with cortex slides of SITR2-WT and SIRT2-KO samples.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cardiaque humain, tissu cérébral de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 43 publications below
• IHC: See 5 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Informations sur le produit

19655-1-AP cible SIRT2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poisson-zèbre, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRT2 Protéine recombinante Ag7756
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 37-45 kDa
• Numéro d’acquisition GenBank: BC003547
• Symbole du gène: SIRT2
• Identification du gène (NCBI): 22933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
human,mouse	WB,IF	Neuron; Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.; Authors - Kelly A Chamberlain ; KO Validated
human	WB	J Clin Invest; SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair.; Authors - Manchao Zhang
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
mouse	WB	Aging Cell; SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.; Authors - Yingying Zhao ; KD Validated

Produits associés

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HDAC6 Polyclonal antibody

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10099-1-AP

FOXO1 Polyclonal antibody

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CDC14B Polyclonal antibody

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HOXA10 Polyclonal antibody

26497-1-AP