Anticorps Monoclonal anti-SIRT2

• Phare
• Validé par KD/KO

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D8G10

N° de cat : 66410-1-PBS

Synonymes

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Galerie de données de validation

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  WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66410-1-Ig (same clone as 66410-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66410-1-Ig (same clone as 66410-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 66410-1-Ig (same clone as 66410-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66410-1-Ig (same clone as 66410-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66410-1-PBS cible SIRT2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT2 Protéine recombinante Ag7756
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 37-45 kDa
• Numéro d’acquisition GenBank: BC003547
• Symbole du gène: SIRT2
• Identification du gène (NCBI): 22933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.

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