Anticorps Recombinant de lapin anti-SIRT3

SIRT3 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2P6

N° de cat : 82210-1-PBS

Synonymes

EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3

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Galerie de données de validation

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  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82210-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82210-1-RR against Human SIRT3 were performed. The affinity constant is 0.261 nM.

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Informations sur le produit

82210-1-PBS cible SIRT3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SIRT3 Protéine recombinante Ag26280
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC001042
• Symbole du gène: SIRT3
• Identification du gène (NCBI): 23410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

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