Anticorps Monoclonal anti-SIRT4

• Phare
• Validé par KD/KO

SIRT4 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

1D5A2

N° de cat : 66543-1-Ig

Synonymes

SIR2 like protein 4, SIR2L4, SIRT4

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Galerie de données de validation

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  WB analysis of HeLa using 66543-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66543-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66543-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66543-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellule NCCIT, cellules L02, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:80000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

66543-1-Ig cible SIRT4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT4 Protéine recombinante Ag17347
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC109319
• Symbole du gène: SIRT4
• Identification du gène (NCBI): 23409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Publications

Species	Application	Title
human	WB	Cell Death Differ; SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.; Authors - Shuai Leng ; KD Validated
human	WB	Cancer Lett; SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.; Authors - Mengqi Liu
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB,IHC,IF	Lab Invest; Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss; Authors - Yi Yi
human	WB	Dis Model Mech; Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.; Authors - Leonie Drews
human	WB	Sci Rep; Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.; Authors - Kalaiselvi Sivalingam