- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-SIRT4
SIRT4 Monoclonal Antibody for WB, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain
Applications
WB, IHC, IF, ELISA
Conjugaison
Non conjugué
CloneNo.
1D5A2
N° de cat : 66543-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules HeLa, cellule NCCIT, cellules L02, cellules PC-3 |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:80000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 34 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
Informations sur le produit
66543-1-Ig cible SIRT4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain
Réactivité | Humain |
Réactivité citée | Humain |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | SIRT4 Protéine recombinante Ag17347 |
Nom complet | sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) |
Masse moléculaire calculée | 314 aa, 35 kDa |
Poids moléculaire observé | 35 kDa |
Numéro d’acquisition GenBank | BC109319 |
Symbole du gène | SIRT4 |
Identification du gène (NCBI) | 23409 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.
Protocole
Product Specific Protocols | |
---|---|
WB protocol for SIRT4 antibody 66543-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer | ||
Cell Death Differ SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.
| ||
Cancer Lett SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer. | ||
iScience Sirt6 regulates the proliferation of neural precursor cells and cortical neurogenesis in mice | ||
Int Immunopharmacol Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma | ||
Lab Invest Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss |