Anticorps Monoclonal anti-SIRT4

• Phare
• Validé par KD/KO

SIRT4 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D5A2

N° de cat : 66543-1-PBS

Synonymes

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4

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Galerie de données de validation

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  WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66543-1-Ig (same clone as 66543-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66543-1-Ig (same clone as 66543-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66543-1-Ig (same clone as 66543-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66543-1-PBS cible SIRT4 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT4 Protéine recombinante Ag17347
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC109319
• Symbole du gène: SIRT4
• Identification du gène (NCBI): 23409
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

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