Anticorps Monoclonal anti-SIRT5

SIRT5 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1F2B2

N° de cat : 67257-1-Ig

Synonymes

1F2B2, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

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Galerie de données de validation

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  WB analysis of HeLa using 67257-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67257-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67257-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67257-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67257-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 67257-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules K-562, cellules L02, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

67257-1-Ig cible SIRT5 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT5 Protéine recombinante Ag7743
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC000126
• Symbole du gène: SIRT5
• Identification du gène (NCBI): 23408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.

Publications

Species	Application	Title
human	WB,IP,IF	Adv Sci (Weinh); SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma; Authors - Qifan Hu
human	WB	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
	WB,IF	Inflammation; Moxibustion Alleviates Inflammation via SIRT5-mediated Post-translational Modification and Macrophage Polarization; Authors - Chuan-Yi Zuo
bovine	WB,IP	Cell Signal; SIRT5-mediated GLS and GDH desuccinylation attenuates the autophagy of bovine mammary epithelial cells induced by ammonia; Authors - Hanlin Yang ; KO Validated
human	WB	Gynecol Endocrinol; SIRT5 regulates granulosa cell proliferation and apoptosis in polycystic ovarian syndrome via desuccinylation of GLI1; Authors - Yiwen Zhang
	WB	Genes Dis; SIRT5 promotes the osteo-inductive potential of BMP9 by stabilizing the HIF-1α protein in mouse embryonic fibroblasts; Authors - Lu Liu

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