Anticorps Recombinant de lapin anti-SIRT5

• Phare
• Validé par KD/KO

SIRT5 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

7L20

N° de cat : 82246-1-RR

Synonymes

7L20, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

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Galerie de données de validation

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  WB analysis using 82246-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82246-1-RR

  WB result of SIRT5 antibody (82246-1-RR; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT5 transfected HEK-293 cells.

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  IF Staining of HeLa using 82246-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  Affinity and Kinetic Characterization of 82246-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HCT 116, cellules HEK-293, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82246-1-RR cible SIRT5 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SIRT5 Protéine recombinante Ag7195
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 32-33 kDa
• Numéro d’acquisition GenBank: BC000126
• Symbole du gène: SIRT5
• Identification du gène (NCBI): 23408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.

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