Anticorps Monoclonal anti-SIRT6

SIRT6 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2H4G8

N° de cat : 67510-1-Ig

Synonymes

SIR2 like protein 6, SIR2L6, SIRT6

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Galerie de données de validation

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  WB analysis using 67510-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67510-1-Ig (SIRT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules JAR, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NCCIT

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67510-1-Ig cible SIRT6 dans les applications de WB, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIRT6 Protéine recombinante Ag24103
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC028220
• Symbole du gène: SIRT6
• Identification du gène (NCBI): 51548
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).

Publications

Species	Application	Title
mouse	WB	Cell Prolif; 20-HETE synthesis inhibition attenuates traumatic brain injury-induced mitochondrial dysfunction and neuronal apoptosis via the SIRT1/PGC-1α pathway: A translational study.; Authors - Wenxing Cui
mouse	WB	ACS Omega; Harnessing the Therapeutic Potential of Ginsenoside Rd for Activating SIRT6 in Treating a Mouse Model of Nonalcoholic Fatty Liver Disease; Authors - Tianqi Cui
mouse	IF,WB,CoIP	Mol Med; Microglia Sirt6 modulates the transcriptional activity of NRF2 to ameliorate high-fat diet-induced obesity; Authors - Xiaoxia Xiao
mouse	WB	J Orthop Res; Orientin inhibits inflammation in chondrocytes and attenuates osteoarthritis through Nrf2/NF-κB and SIRT6/NF-κB pathway; Authors - Weiyi Xia