Anticorps Polyclonal de lapin anti-SIRT7

SIRT7 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 29729-1-AP

Synonymes

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

---

Galerie de données de validation

• 

  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 29729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIRT7 antibody (29729-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HCT 116, cellules HeLa, cellules MCF-7
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

29729-1-AP cible SIRT7 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRT7 Protéine recombinante Ag31794
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Masse moléculaire calculée: 45KD
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_016538
• Symbole du gène: SIRT7
• Identification du gène (NCBI): 51547
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publications

Species	Application	Title
mouse	WB,CoIP	Acta Pharmacol Sin; JOSD2 alleviates acute kidney injury through deubiquitinating SIRT7 and negativity regulating SIRT7-NF-κB inflammatory pathway in renal tubular epithelial cells; Authors - Ying Zhao ; KD Validated
human,rat	WB,IHC	Int Urol Nephrol; SIRT7 silencing by miR-152-3p confers cell apoptosis and renal functional impairment induced by renal ischaemia/reperfusion injury; Authors - Yan Wang

{{ptg:RelatedPrimaryAntibodies}}