Anticorps Monoclonal anti-SIX1

SIX1 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D7D1

N° de cat : 67741-1-PBS

Synonymes

2D7D1, BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1

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Galerie de données de validation

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  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67741-1-Ig (same clone as 67741-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67741-1-PBS cible SIX1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIX1 Protéine recombinante Ag28570
• Nom complet: SIX homeobox 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC008874
• Symbole du gène: SIX1
• Identification du gène (NCBI): 6495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

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