Anticorps Monoclonal anti-SIX2

SIX2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1F9G11

N° de cat : 66347-1-Ig

Synonymes

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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Galerie de données de validation

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  WB analysis using 66347-1-Ig

  Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66347-1-Ig

  Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66347-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66347-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66347-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDCK using 66347-1-Ig

  MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 66347-1-Ig

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66347-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse embryo using 66347-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 66347-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66347-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIX2 antibody (66347-1-Ig, Clone: 1F9G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de porc, cellules HEK-293, cellules L02, cellules MDCK, cellules NCCIT, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu embryonnaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

66347-1-Ig cible SIX2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIX2 Protéine recombinante Ag2124
• Nom complet: SIX homeobox 2
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC024033
• Symbole du gène: SIX2
• Identification du gène (NCBI): 10736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

Publications

Species	Application	Title
mouse	IF	Development; Aberrant centrosome biogenesis disrupts nephron and collecting duct progenitor growth and fate resulting in fibrocystic kidney disease; Authors - Tao Cheng
mouse	IF	Biochem Biophys Res Commun; Disruption of Gen1 causes ectopic budding and kidney hypoplasia in mice; Authors - Yaxin Li
mouse	IF	bioRxiv; Engineering kidney developmental trajectory using culture boundary conditions; Authors - Aria Zheyuan Huang
mouse	IHC	Dev Dyn; Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling; Authors - Wen Shi
mouse	IHC	Nucleic Acids Res; The transcriptional coactivator Eya1 exerts transcriptional repressive activity by interacting with REST corepressors and REST-binding sequences to maintain nephron progenitor identity; Authors - Jun Li
mouse	WB,IF	J Am Soc Nephrol; Chromatin Remodelers Interact with Eya1 and Six2 to Target Enhancers to Control Nephron Progenitor Cell Maintenance; Authors - Jun Li

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