Anticorps Monoclonal anti-SIX2

SIX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9G11

N° de cat : 66347-1-PBS

Synonymes

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

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Galerie de données de validation

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  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

  Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66347-1-Ig (same clone as 66347-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66347-1-Ig (same clone as 66347-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66347-1-Ig (same clone as 66347-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 66347-1-Ig (same clone as 66347-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 66347-1-Ig (same clone as 66347-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66347-1-Ig (same clone as 66347-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIX2 antibody (66347-1-Ig, Clone: 1F9G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66347-1-PBS cible SIX2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIX2 Protéine recombinante Ag2124
• Nom complet: SIX homeobox 2
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC024033
• Symbole du gène: SIX2
• Identification du gène (NCBI): 10736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

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