Anticorps Monoclonal anti-SIX2

SIX2 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

canin, Humain, porc, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1F9G11

N° de cat : CL594-66347

Synonymes

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2

---

Galerie de données de validation

• 

  IF Staining of HeLa using CL594-66347

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 SIX2 antibody (CL594-66347, Clone: 1F9G11 ) at dilution of 1:1200, CL488-Phalloidin (green).

• 

  FC experiment of C2C12 using CL594-66347

  1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules C2C12

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:600-1:2400
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-66347 cible SIX2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons canin, Humain, porc, rat, souris

• Réactivité: canin, Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SIX2 Protéine recombinante Ag2124
• Nom complet: SIX homeobox 2
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC024033
• Symbole du gène: SIX2
• Identification du gène (NCBI): 10736
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

{{ptg:RelatedPrimaryAntibodies}}