Anticorps Polyclonal de lapin anti-SKIL

• Phare
• Validé par KD/KO

SKIL Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19218-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19218-1-AP (same clone as 19218-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 19218-1-AP (same clone as 19218-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 19218-1-AP (same clone as 19218-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  IP result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 19218-1-AP (same clone as 19218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19218-1-AP (same clone as 19218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19218-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19218-1-PBS cible SKIL dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKIL Protéine recombinante Ag5282
• Nom complet: SKI-like oncogene
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC059386
• Symbole du gène: SKIL
• Identification du gène (NCBI): 6498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

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