Anticorps Monoclonal anti-SKIV2L

SKIV2L Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A9G2

Avis (2)

N° de cat : 68547-1-Ig

Synonymes

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

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Galerie de données de validation

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  WB analysis using 68547-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 68547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 68547-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68547-1-Ig cible SKIV2L dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SKIV2L Protéine recombinante Ag33679
• Nom complet: superkiller viralicidic activity 2-like (S. cerevisiae)
• Masse moléculaire calculée: 1246 aa, 138 kDa
• Poids moléculaire observé: 138-145 kDa
• Numéro d’acquisition GenBank: BC015758
• Symbole du gène: SKIV2L
• Identification du gène (NCBI): 6499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Specific detection of human Skiv2l in RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

SKIV2L Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:68547-1-Ig)

FH

Tsimafei (Verified Customer) (11-19-2024)

Antibody detect a band of the correct molecular weight in U2OS cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: U2OS

SKIV2L Antibody Western Blot validation (1:3000 dilution) in U2OS (Cat no:68547-1-Ig)

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