Anticorps Monoclonal anti-SKIV2L

SKIV2L Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A9G2

N° de cat : 68547-1-PBS

Synonymes

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

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Galerie de données de validation

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  WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68547-1-PBS cible SKIV2L dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SKIV2L Protéine recombinante Ag33679
• Nom complet: superkiller viralicidic activity 2-like (S. cerevisiae)
• Masse moléculaire calculée: 1246 aa, 138 kDa
• Poids moléculaire observé: 138-145 kDa
• Numéro d’acquisition GenBank: BC015758
• Symbole du gène: SKIV2L
• Identification du gène (NCBI): 6499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

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