Anticorps Polyclonal de lapin anti-SKIV2L2

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (2)

N° de cat : 12719-2-AP

Synonymes

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

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Galerie de données de validation

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  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12719-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12719-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12719-2-AP

  IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug.

• 

  IHC staining of human stomach using 12719-2-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse testis using 12719-2-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SKIV2L2 antibody (12719-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

12719-2-AP cible SKIV2L2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKIV2L2 Protéine recombinante Ag3414
• Nom complet: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Masse moléculaire calculée: 1042 aa, 118 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC028604
• Symbole du gène: SKIV2L2
• Identification du gène (NCBI): 23517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IP	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan
human	WB	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang
		Nucleic Acids Res; The NEXT complex regulates H3K27me3 levels to affect cancer progression by degrading G4/U-rich lncRNAs; Authors - Qianqian Yang
human	WB,IHC	Cell Death Differ; hMTR4 promotes p53 protein degradation and tumor growth by accelerating rRNA processing and regulating the RPL5-MDM2 axis; Authors - Chen Xie ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (05-09-2025)

Works very well in western blot (1:1000 dilution) (tested on nuclear cell extracts)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

SKIV2L2 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:12719-2-AP)

FH

Roy (Verified Customer) (06-12-2024)

Great antibody to detect MTR4/SKIV2L2 from HeLa/Jurkat cell extracts by WB (1/1000 - 1h incubation at RT) and did work well to immunoprecipitate MTR4/SKIV2L2 (4µg/IP).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa/Jurkat

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