Anticorps Polyclonal de lapin anti-SKIV2L2

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12719-2-PBS

Synonymes

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

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Galerie de données de validation

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  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12719-2-AP (same clone as 12719-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 12719-2-AP (same clone as 12719-2-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 12719-2-AP (same clone as 12719-2-PBS)

  IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug. This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12719-2-AP (same clone as 12719-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 12719-2-AP (same clone as 12719-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SKIV2L2 antibody (12719-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12719-2-PBS in a different storage buffer formulation.

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Informations sur le produit

12719-2-PBS cible SKIV2L2 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKIV2L2 Protéine recombinante Ag3414
• Nom complet: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Masse moléculaire calculée: 1042 aa, 118 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC028604
• Symbole du gène: SKIV2L2
• Identification du gène (NCBI): 23517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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