Anticorps Recombinant de lapin anti-SKIV2L2

SKIV2L2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251430D11

N° de cat : 86536-1-RR

Synonymes

ATP dependent helicase SKIV2L2, ATP-dependent RNA helicase DOB1, ATP-dependent RNA helicase SKIV2L2, DOB1, EC:3.6.4.13

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Galerie de données de validation

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  WB analysis using 86536-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86536-1-RR (SKIV2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 86536-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIV2L2 antibody (86536-1-RR, Clone: 251430D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86536-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86536-1-RR against Human SKIV2L2 were performed. The affinity constant is 0.198 nM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules Jurkat, cellules Neuro-2a, tissu testiculaire de souris
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86536-1-RR cible SKIV2L2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SKIV2L2 Protéine recombinante Ag3414
• Nom complet: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Masse moléculaire calculée: 1042 aa, 118 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC028604
• Symbole du gène: SKIV2L2
• Identification du gène (NCBI): 23517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SKIV2L2 (Ski2-like RNA helicase 2, also called Mtr4) is an evolutionarily conserved 3′→5′ RNA helicase belonging to the Ski2-like subfamily of DExH-box helicases.SKIV2L2 is an essential component of the nuclear exosome targeting (NEXT) complex, where it acts as the major ATP-dependent motor that threads RNA substrates into the catalytic core of the RNA exosome for processing and degradation. Through this activity, SKIV2L2 regulates the turnover of aberrant pre-mRNAs, non-coding RNAs, and promoter-associated RNAs, thereby influencing gene expression, ribosomal RNA biogenesis, and maintenance of nuclear RNA homeostasis. It plays a role in cell differentiation and spermatogenesis. Clinically, germline mutations in SKIV2L2 have been associated with tricho-hepato-enteric syndrome, highlighting its importance in intestinal and hepatic development. (PMID: 39863862)

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