Anticorps Polyclonal de lapin anti-SKP2

• Phare
• Validé par KD/KO

SKP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15010-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15010-1-AP (same clone as 15010-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  WB result of SKP2 antibody (15010-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  WB result of SKP2 antibody (15010-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SKP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 15010-1-AP (same clone as 15010-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15010-1-AP (SKP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15010-1-AP (same clone as 15010-1-PBS)

  IP result of anti-SKP2 (IP:15010-1-AP, 4ug; Detection:15010-1-AP 1:300) with HEK-293 cells lysate 2440ug. This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15010-1-AP (SKP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15010-1-AP (SKP2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 15010-1-AP (same clone as 15010-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15010-1-AP (SKP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15010-1-AP (same clone as 15010-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (15010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15010-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15010-1-PBS cible SKP2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKP2 Protéine recombinante Ag6826
• Nom complet: S-phase kinase-associated protein 2 (p45)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 46-48 kDa
• Numéro d’acquisition GenBank: BC001441
• Symbole du gène: SKP2
• Identification du gène (NCBI): 6502
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

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