Anticorps Polyclonal de lapin anti-SLAMF7/CD319

SLAMF7/CD319 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12905-1-AP

Synonymes

SLAMF7, Membrane protein FOAP-12, FOAP-12, CS1, CRACC

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Galerie de données de validation

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  WB analysis using 12905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7/CD319 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 12905-1-AP

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12905-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 12905-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12905-1-AP (SLAMF7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12905-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12905-1-AP (SLAMF7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: IM-9 cells, cellules Jurkat, tissu splénique de porc, tissu splénique humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12905-1-AP cible SLAMF7/CD319 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLAMF7/CD319 Protéine recombinante Ag3804
• Nom complet: SLAM family member 7
• Masse moléculaire calculée: 335 aa, 37 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC027867
• Symbole du gène: SLAMF7
• Identification du gène (NCBI): 57823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocyte activation molecule (SLAM) family receptors is a group of type I transmembrane receptors belonging to the immunoglobulin superfamily (PMID: 37251372). The SLAM family contains nine members that contain an extracellular segment comprising two or four Ig-like domains (V-like variable and C2-like constant), a transmembrane region and a cytoplasmic tail (PMID: 29662641). They are involved in various physiological and pathological processes, including the regulation of immunological responses and the route of viral entry. SLAMF7, also known as CS1, CRACC or CD319, is expressed on NK cells, CD8+ T lymphocytes, B lymphocytes, and mature dendritic cells (PMID: 28861320). It exerts both activating and inhibitory effects depending on the expression status of SAP or EAT-2. SLAMF7 is overexpressed in multiple myeloma and makes it a target for immunotherapy (PMID: 28861320). The native molecular weight of SLAMF7 is 37 kDa and the apparent molecular mass shifts due to glycosylation (PMID: 11698418; 36381324).

Publications

Species	Application	Title
human	WB	Int J Chron Obstruct Pulmon Dis; Analysis of Key Genes and miRNA-mRNA Networks Associated with Glucocorticoids Treatment in Chronic Obstructive Pulmonary Disease; Authors - Jian-Jun Wu
mouse	WB,IF	Exploration (Beijing); Bioengineer mesenchymal stem cell for treatment of glioma by IL-12 mediated microenvironment reprogramming and nCD47-SLAMF7 mediated phagocytosis regulation of macrophages; Authors - Man Li

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