Anticorps Recombinant de lapin anti-SLAMF7/CD319

SLAMF7/CD319 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241320B6

N° de cat : 84254-1-RR

Synonymes

SLAMF7, 241320B6, CD2-like receptor-activating cytotoxic cells, CD319, CRACC

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Galerie de données de validation

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  WB analysis using 84254-1-RR

  IM-9 cells were subjected to SDS PAGE followed by western blot with 84254-1-RR (SLAMF7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84254-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84254-1-RR (SLAMF7/CD319 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84254-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84254-1-RR (SLAMF7/CD319 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of IM-9 using 84254-1-RR

  Immunofluorescent analysis of (4% PFA) fixed IM-9 cells using SLAMF7/CD319 antibody (84254-1-RR, Clone: 241320B6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84254-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84254-1-RR against Human SLAMF7/CD319 were performed. The affinity constant is 1.31 nM.

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Applications testées

• Résultats positifs en WB: IM-9 cells,
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: IM-9 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84254-1-RR cible SLAMF7/CD319 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: SLAM family member 7
• Masse moléculaire calculée: 335 aa, 37 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC027867
• Symbole du gène: SLAMF7
• Identification du gène (NCBI): 57823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocyte activation molecule (SLAM) family receptors is a group of type I transmembrane receptors belonging to the immunoglobulin superfamily (PMID: 37251372). The SLAM family contains nine members that contain an extracellular segment comprising two or four Ig-like domains (V-like variable and C2-like constant), a transmembrane region and a cytoplasmic tail (PMID: 29662641). They are involved in various physiological and pathological processes, including the regulation of immunological responses and the route of viral entry. SLAMF7, also known as CS1, CRACC or CD319, is expressed on NK cells, CD8+ T lymphocytes, B lymphocytes, and mature dendritic cells (PMID: 28861320). It exerts both activating and inhibitory effects depending on the expression status of SAP or EAT-2. SLAMF7 is overexpressed in multiple myeloma and makes it a target for immunotherapy (PMID: 28861320). The native molecular weight of SLAMF7 is 37 kDa and the apparent molecular mass shifts due to glycosylation (PMID: 11698418; 36381324).

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