Anticorps Polyclonal de lapin anti-NKCC1/SLC12A2

• Phare
• Validé par KD/KO

NKCC1/SLC12A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(31)

Avis (2)

N° de cat : 13884-1-AP

Synonymes

NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter

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Galerie de données de validation

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  WB analysis using 13884-1-AP

  Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13884-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 13884-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1/SLC12A2 antibody (13884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de souris incubé à 37 °C
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 9 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

13884-1-AP cible NKCC1/SLC12A2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKCC1/SLC12A2 Protéine recombinante Ag4991
• Nom complet: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC033003
• Symbole du gène: NKCC1/SLC12A2
• Identification du gène (NCBI): 6558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Publications

Species	Application	Title
mouse	WB,IHC	Science; A causative link between inner ear defects and long-term striatal dysfunction.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; Early uneven ear input induces long-lasting differences in left-right motor function.; Authors - Michelle W Antoine
mouse	IF	Front Immunol; Myeloid-derived growth factor promotes M2 macrophage polarization and attenuates Sjögren's syndrome via suppression of the CX3CL1/CX3CR1 axis; Authors - Zi Yang
mouse	IHC	PLoS Biol; The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.; Authors - Krisztina Tóth
mouse	WB,IHC	J Neurosci; The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety.; Authors - Michelle W Antoine
mouse	IF	Front Mol Neurosci; Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampus; Authors - Fabio Cesar Tescarollo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (11-05-2024)

Not sure the marker is actually a positive marker and not a result of the autofluorescence. NKCC1 is a chloride cotransporter that should not be expressed in a fully developed cortex, but it should appear in focal cortical dysplasia tissue used for this assay.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

NKCC1/SLC12A2 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:13884-1-AP)

FH

Valentina (Verified Customer) (02-01-2023)

Multiple bands were detectable around 100 - 150 kDa, with different protocols and gels. The antibody is definitely working even if not optimally, probably necessary to try with a higher dilution and a lower amount of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Choroid plexus homogenates

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