Anticorps Polyclonal de lapin anti-NKCC1/SLC12A2

NKCC1/SLC12A2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 28055-1-AP

Synonymes

NKCC1,SLC12A2, NKCC1, SLC12A2, Basolateral Na K Cl symporter, Basolateral Na-K-Cl symporter

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Galerie de données de validation

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  WB analysis using 28055-1-AP

  Boiled and unboiled HeLa cell lysates were subjected to SDS PAGE followed by western blot with 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28055-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28055-1-AP (NKCC1/SLC12A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 28055-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NKCC1/SLC12A2 antibody (28055-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 28055-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using NKCC1,SLC12A2 antibody (28055-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa non bouillies,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28055-1-AP cible NKCC1/SLC12A2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NKCC1/SLC12A2 Protéine recombinante Ag27895
• Nom complet: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC033003
• Symbole du gène: NKCC1/SLC12A2
• Identification du gène (NCBI): 6558
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

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