Anticorps Recombinant de lapin anti-SLC12A8

SLC12A8 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240082B11

N° de cat : 83225-2-PBS

Synonymes

CCC9, Cation-chloride cotransporter 9, 240082B11

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Galerie de données de validation

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  IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR (same clone as 83225-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 83225-2-RR (same clone as 83225-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83225-2-RR (same clone as 83225-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00171-1

  Cytometric bead array standard curve of MP00171-1, SLC12A8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83225-1-PBS. Detection antibody: 83225-2-PBS. Standard: Ag34354. Range: 5-40 ng/mL.

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  Affinity and Kinetic Characterization of 83225-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

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Informations sur le produit

83225-2-PBS cible SLC12A8 dans les applications de IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC12A8 Protéine recombinante Ag34354
• Nom complet: solute carrier family 12 (potassium/chloride transporters), member 8
• Masse moléculaire calculée: 714 aa, 80 kDa
• Numéro d’acquisition GenBank: BC020506
• Symbole du gène: SLC12A8
• Identification du gène (NCBI): 84561
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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