Anticorps Recombinant de lapin anti-VGLUT2

VGLUT2 Recombinant Antibody for WB, IF-Fro, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-Fro, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241255B5

N° de cat : 84083-2-PBS

Synonymes

SLC17A6, 241255B5, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI

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Galerie de données de validation

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  WB analysis using 84083-2-RR (same clone as 84083-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using VGLUT2 antibody (84083-2-RR, Clone: 241255B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00998-1

  Cytometric bead array standard curve of MP00998-1, SLC17A6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84083-1-PBS. Detection antibody: 84083-2-PBS. Standard: Ag29565. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84083-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84083-2-RR against Human VGLUT2 were performed. The affinity constant is 1.48 nM.

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Informations sur le produit

84083-2-PBS cible VGLUT2 dans les applications de WB, IF-Fro, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: VGLUT2 Protéine recombinante Ag29565
• Nom complet: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_020346
• Symbole du gène: VGLUT2
• Identification du gène (NCBI): 57084
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

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