Anticorps Polyclonal de lapin anti-VGLUT1

VGLUT1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(21)

N° de cat : 55491-1-AP

Synonymes

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

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Galerie de données de validation

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  WB analysis using 55491-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55491-1-AP (VGLUT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55491-1-AP (VGLUT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55491-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55491-1-AP (VGLUT1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 55491-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 55491-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using VGLUT1 antibody (55491-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of SH-SY5Y using 55491-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human VGLUT1 (55491-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris non bouilli,
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:750-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 18 publications below

Informations sur le produit

55491-1-AP cible VGLUT1 dans les applications de WB, IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Masse moléculaire calculée: 62 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: NM_020309
• Symbole du gène: VGLUT1
• Identification du gène (NCBI): 57030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.

Publications

Species	Application	Title
mouse	IF	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	IF	PLoS Biol; The anterior cingulate cortex controls the hyperactivity in subthalamic neurons in male mice with comorbid chronic pain and depression; Authors - Ying-Di Wang
human	IF	iScience; Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons; Authors - Waka Lin
human	IF	Neurobiol Dis; GRN-/- iPSC-derived cortical neurons recapitulate the pathological findings of both frontotemporal lobar degeneration and neuronal ceroidolipofuscinosis; Authors - Patrizia Bossolasco
mouse	IF	J Neurosci; Insula→Amygdala and Insula→Thalamus Pathways Are Involved in Comorbid Chronic Pain and Depression-Like Behavior in Mice; Authors - Jing Chen
rat	IF	Neurobiol Dis; PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by influencing synaptic function in the primary motor cortex of rats.; Authors - Juan Mo

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