Anticorps Recombinant de lapin anti-SLC17A9

SLC17A9 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240328H7

N° de cat : 83505-1-PBS

Synonymes

Voltage-gated purine nucleotide uniporter SLC17A9, VNUT, Vesicular nucleotide transporter, FLJ23412, C20orf59

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Galerie de données de validation

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  WB analysis using 83505-1-RR (same clone as 83505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83505-1-RR (SLC17A9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 83505-1-RR (same clone as 83505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83505-1-RR (SLC17A9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  FC experiment of Caco-2 using 83505-1-RR (same clone as 83505-1-PBS)

  1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SLC17A9 Recombinant antibody (83505-1-RR, Clone:240328H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83505-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83505-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83505-1-RR against Human SLC17A9 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83505-1-PBS cible SLC17A9 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC17A9 Protéine recombinante Ag24970
• Nom complet: solute carrier family 17, member 9
• Masse moléculaire calculée: 436 aa, 47 kDa
• Poids moléculaire observé: 50 kD
• Numéro d’acquisition GenBank: BC025312
• Symbole du gène: SLC17A9
• Identification du gène (NCBI): 63910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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