Anticorps Polyclonal de lapin anti-SLC19A3

SLC19A3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (2)

N° de cat : 13407-1-AP

Synonymes

SLC19A3, Thiamine transporter 2, ThTr 2, THTR2

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Galerie de données de validation

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  WB analysis using 13407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13407-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13407-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13407-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13407-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13407-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13407-1-AP

  IP result of anti-SLC19A3 (IP:13407-1-AP, 4ug; Detection:13407-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules 3T3-L1, cellules SH-SY5Y, tissu cardiaque de rat, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu placentaire humain, tissu rénal de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 1 publications below

Informations sur le produit

13407-1-AP cible SLC19A3 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC19A3 Protéine recombinante Ag4053
• Nom complet: solute carrier family 19, member 3
• Masse moléculaire calculée: 496 aa, 56 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC032014
• Symbole du gène: SLC19A3
• Identification du gène (NCBI): 80704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB	Sci Transl Med; CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.; Authors - Sara Picó
human	WB	Nat Commun; Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia; Authors - Alaullah Sheikh
human	WB	Front Cell Dev Biol; Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.; Authors - Jane L Tarry-Adkins
human	WB,IF	J Nutr Biochem; Up-regulation of vitamin B1 homeostasis genes in breast cancer.; Authors - Zastre Jason A JA
human	WB	J Biol Chem; Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.; Authors - Subrata Sabui
mouse	WB	Front Pharmacol; Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice.; Authors - Hong Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Valliammai (Verified Customer) (02-15-2023)

Results are highly reliable. Thanks for the service.

• Applications: Western Blot, Immunofluorescence, Protein purification

FH

Selvaraj (Verified Customer) (12-20-2022)

We are frequently using this antibody in our lab

• Applications: Western Blot
• Cell Tissue Type: Human intestinal cell lines

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