Anticorps Polyclonal de lapin anti-EAAT4

EAAT4 Polyclonal Antibody for WB, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12876-1-AP

Synonymes

SLC1A6, Sodium-dependent glutamate/aspartate transporter

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Galerie de données de validation

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  WB analysis of A375 using 12876-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cerebellum using 12876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of mouse cerebellum using 12876-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse cerebellum tissue using EAAT4 antibody (12876-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules A375
• Résultats positifs en IF-Fro: tissu de cervelet de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

12876-1-AP cible EAAT4 dans les applications de WB, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EAAT4 Protéine recombinante Ag3554
• Nom complet: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
• Masse moléculaire calculée: 61.6 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC028721
• Symbole du gène: EAAT4
• Identification du gène (NCBI): 6511
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice.; Authors - Michael Rabenstein
human	WB	Neoplasia; RUNX2 enhances bladder cancer progression by promoting glutamine metabolism; Authors - Zhigang Huang
human	WB	Cancer Immunol Immunother; Glutamate transporter SLC1A6 promotes resistance to immunotherapy in cancer; Authors - Chenchen Li

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