Anticorps Polyclonal de lapin anti-SLC20A1

• Phare
• Validé par KD/KO

SLC20A1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12423-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12423-1-AP (same clone as 12423-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12423-1-AP (same clone as 12423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12423-1-AP (same clone as 12423-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 12423-1-AP (same clone as 12423-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

  IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12423-1-PBS cible SLC20A1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC20A1 Protéine recombinante Ag3084
• Nom complet: solute carrier family 20 (phosphate transporter), member 1
• Masse moléculaire calculée: 679 aa, 74 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC019944
• Symbole du gène: SLC20A1
• Identification du gène (NCBI): 6574
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

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