Anticorps Polyclonal de lapin anti-URAT1

• Phare
• Validé par KD/KO

URAT1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(93)

N° de cat : 14937-1-AP

Synonymes

SLC22A12, RST, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein

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Galerie de données de validation

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  WB analysis using 14937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 14937-1-AP

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 14937-1-AP (URAT1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14937-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14937-1-AP (URAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu rénal de porc, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 85 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Informations sur le produit

14937-1-AP cible URAT1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: URAT1 Protéine recombinante Ag6770
• Nom complet: solute carrier family 22 (organic anion/urate transporter), member 12
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 65-70 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC053348
• Symbole du gène: URAT1
• Identification du gène (NCBI): 116085
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice; Authors - Xiao-Li Guo
human,mouse	WB	Phytomedicine; The inhibitory effect of Astragalus flavone extract on hyperuricemia and its underlying molecular mechanism by targeting JNK/AP-1/NLRP3/IL-1β signaling pathway; Authors - Hongjuan Xia
rat	WB	Phytomedicine; Mechanistic insights into the potentiation and toxicity mitigation of myocardial infarction treatment with salvianolate and ticagrelor; Authors - Yan Wan
mouse	WB	Mol Metab; URAT1-selective inhibition ameliorates insulin resistance by attenuating diet-induced hepatic steatosis and brown adipose tissue whitening in mice.; Authors - Yoshiro Tanaka
mouse	IHC	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
mouse	WB	Int J Mol Sci; Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis.; Authors - Yiming Wang

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