Anticorps Monoclonal anti-SLC25A12

SLC25A12 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2B10

N° de cat : 67467-1-PBS

Synonymes

AGC1, Araceli hiperlarga, Aralar, Aralar1, Electrogenic aspartate/glutamate antiporter SLC25A12, mitochondrial

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Galerie de données de validation

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  WB analysis of LNCaP using 67467-1-Ig (same clone as 67467-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67467-1-Ig (same clone as 67467-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 67467-1-Ig (same clone as 67467-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 67467-1-Ig (SLC25A12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67467-1-Ig (same clone as 67467-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67467-1-Ig (SLC25A12 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67467-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50650-2

  Cytometric bead array standard curve of MP50650-2, SLC25A12 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67467-4-PBS. Detection antibody: 67467-1-PBS. Standard:Ag13576. Range: 1.563-100 ng/mL.

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Informations sur le produit

67467-1-PBS cible SLC25A12 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC25A12 Protéine recombinante Ag13576
• Nom complet: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Masse moléculaire calculée: 75 kDa, 63 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC016932
• Symbole du gène: SLC25A12
• Identification du gène (NCBI): 8604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A12, also known as ARALAR1, is a key component of the malate-aspartate shuttle, a metabolic system that facilitates the transfer of reducing equivalents (NADH) from the cytoplasm into mitochondria, thereby supporting oxidative phosphorylation and ATP production. SLC25A12 dysfunction results in impaired mitochondrial energy production, which can lead to neuronal damage, developmental delays, and myelination defects (PMID: 20015484).

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