Anticorps Recombinant de lapin anti-SLC25A22

SLC25A22 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250788B11

N° de cat : 86163-1-RR

Synonymes

GC 1, GC1, GC-1, Glutamate/H(+) symporter 1, NET44

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Galerie de données de validation

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  WB analysis using 86163-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86163-1-RR (SLC25A22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86163-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86163-1-RR against Human SLC25A22 were performed. The affinity constant is 1.13 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86163-1-RR cible SLC25A22 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC25A22 Protéine recombinante Ag19958
• Nom complet: solute carrier family 25 (mitochondrial carrier: glutamate), member 22
• Masse moléculaire calculée: 323 aa, 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC023545
• Symbole du gène: SLC25A22
• Identification du gène (NCBI): 79751
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

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