Anticorps Recombinant de lapin anti-ANT1/2

ANT1/2 Recombinant Antibody for WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230247B1

N° de cat : CL750-83104

Synonymes

SLC25A5, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2

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Galerie de données de validation

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  WB analysis using CL750-83104

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83104 (SLC25A5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  FC experiment of PC-3 using CL750-83104

  1x10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody (CL750-83104, Clone:230247B1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules Neuro-2a, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en FC (Intra): cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-83104 cible ANT1/2 dans les applications de WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ANT1/2 Protéine recombinante Ag11956
• Nom complet: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
• Masse moléculaire calculée: 298 aa, 33 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC056160
• Symbole du gène: SLC25A5
• Identification du gène (NCBI): 292
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

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