Anticorps Polyclonal de lapin anti-SLC26A3

SLC26A3 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 13165-1-AP

Synonymes

DRA, Down-regulated in adenoma, CLD, Chloride anion exchanger

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Galerie de données de validation

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  IHC staining of human colon cancer using 13165-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13165-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13165-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13165-1-AP (SLC26A3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu testiculaire de souris, tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13165-1-AP cible SLC26A3 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC26A3 Protéine recombinante Ag3816
• Nom complet: solute carrier family 26, member 3
• Masse moléculaire calculée: 764 aa, 85 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC025671
• Symbole du gène: SLC26A3
• Identification du gène (NCBI): 1811
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC26A3 (Chloride anion exchanger), also known as DRA. It is expected to be located in the cytoplasm and cell membranes. It is a transmembrane glycoprotein, which is mainly located in the lower digestive tract mucosa, especially in the apical membrane of columnar epithelium and some goblet cells. The expression level of SLC26A3 in intestine is different along different parts of intestine, and there are also differences on crypt-villus axis. It is mainly expressed in the apical membrane of colon epithelial cells, less in jejunum and ileum, but not in esophagus. It participates in the absorption of electrically neutral NaCl in intestine and works with Na+/H+ exchanger. Cl-/HCO3- exchange mediated by SLC26A3 is very important for maintaining intestinal acid-base balance. In duodenum, the physiological function of SLC26A3 is related to HCO3- secretion, which plays a central role in neutralizing gastric acid (PMID: 32989468). The molecular weight of SLC26A3 is 84 kDa.

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