Anticorps Recombinant de lapin anti-SLC30A1

SLC30A1 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230152E10

N° de cat : 82895-2-PBS

Synonymes

ZnT-1, ZNT1, Zinc transporter 1, 230152E10

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Galerie de données de validation

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  WB analysis using 82895-2-RR (same clone as 82895-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82895-2-RR (SLC30A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82895-2-RR (same clone as 82895-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug SLC30A1 Recombinant antibody (82895-2-RR, Clone:230152E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82895-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82895-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82895-2-RR against Human SLC30A1 were performed. The affinity constant is 1.49 nM.

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Informations sur le produit

82895-2-PBS cible SLC30A1 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC30A1 Protéine recombinante Ag18567
• Nom complet: solute carrier family 30 (zinc transporter), member 1
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 63~75 kDa
• Numéro d’acquisition GenBank: BC121015
• Symbole du gène: SLC30A1
• Identification du gène (NCBI): 7779
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319).

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