Anticorps Recombinant de lapin anti-SLC30A2

SLC30A2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240405E3

N° de cat : 83666-1-RR

Synonymes

ZnT-2, ZNT2, ZnT 2, Proton-coupled zinc antiporter SLC30A2, PP12488

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Galerie de données de validation

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  WB analysis using 83666-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83666-1-RR (SLC30A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83666-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SLC30A2 Recombinant antibody (83666-1-RR, Clone:240405E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83666-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83666-1-RR against Human SLC30A2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules Caco-2, cellules HEK-293, cellules HeLa, cellules MCF-7, cellules NCCIT
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83666-1-RR cible SLC30A2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC30A2 Protéine recombinante Ag8232
• Nom complet: solute carrier family 30 (zinc transporter), member 2
• Masse moléculaire calculée: 323 aa, 35 kDa
• Poids moléculaire observé: 35 kDa and 52 kDa
• Numéro d’acquisition GenBank: BC006251
• Symbole du gène: SLC30A2
• Identification du gène (NCBI): 7780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and function as a homo- or hetero-dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 kDa (short isoform) and 41 kDa (long isoform). This antibody detects 2 bands of 35 kDa and 52 kDa ( glycosylation form of the long isoform) (PMID: 14608047, PMID: 28174721).

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