Anticorps Recombinant de lapin anti-SLC31A1

• Phare
• Validé par KD/KO

SLC31A1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240868D10

N° de cat : 83844-5-PBS

Synonymes

COPT1, 240868D10, CTR1, Truncated CTR1 form

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Galerie de données de validation

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  WB analysis of HeLa using 83844-5-RR (same clone as 83844-5-PBS)

  WB result of SLC31A1 antibody (83844-5-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells. This data was developed using the same antibody clone with 83844-5-PBS in a different storage buffer formulation.

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  WB analysis of SW480 using 83844-5-RR (same clone as 83844-5-PBS)

  WB result of SLC31A1 antibody (83844-5-RR; 1:800; room temperature for 1.5 hours) with wild-type and SLC31A1 knockout SW480 cells. This data was developed using the same antibody clone with 83844-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 83844-5-RR (same clone as 83844-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83844-5-RR (SLC31A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83844-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83844-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83844-5-RR against Human SLC31A1 were performed. The affinity constant is 3.33 nM.

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Informations sur le produit

83844-5-PBS cible SLC31A1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC31A1 Protéine recombinante Ag26601
• Nom complet: solute carrier family 31 (copper transporters), member 1
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21-23 kDa
• Numéro d’acquisition GenBank: BC013611
• Symbole du gène: SLC31A1
• Identification du gène (NCBI): 1317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC31A1, also known as CTR1, COPT1 and solute carrier family 31 member 1, is a high-affinity copper transporter found in the cell membrane of human cells. It plays a crucial role in maintaining copper homeostasis within the body and functions as a homotrimer to influence dietary copper absorption in the cell membrane (PMID: 37853210; 28507097). SLC31A1 has three putative transmembrane domains, with an N-terminus located extracellularly and a C-terminus located intracellularly (PMID: 12827356). SLC31A1 can be detected a a 28 kDa band corresponding to glycosylated hCTR1 monomer, and a 24 kDa band that is unglycosylated hCTR1. In some membrane preparations a C-terminal degradation product of 17 kDa is also seen (PMID: 16135512). Additionally, SLC31A1 may also resolve as a 30 to 35 kDa and a 60 to 70 kDa protein on immunoblots, forming oligomers corresponding to the expected size of a homotrimeric complex (PMID: 12827356).

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