Anticorps Recombinant de lapin anti-SLC35B4

SLC35B4 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240332F10

N° de cat : 83527-3-RR

Synonymes

YEA4, YEA, UDP-xylose and UDP-N-acetylglucosamine transporter, PSEC0055, Nucleotide sugar transporter SLC35B4

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Galerie de données de validation

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  WB analysis of DU 145 using 83527-3-RR

  DU 145 cells were subjected to SDS PAGE followed by western blot with 83527-3-RR (SLC35B4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 83527-3-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Slc35b4 Recombinant Antibody (83527-3-RR, Clone:240332F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83527-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83527-3-RR against Human SLC35B4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules DU 145,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

83527-3-RR cible SLC35B4 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC35B4 Protéine recombinante Ag22669
• Nom complet: solute carrier family 35, member B4
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC008413
• Symbole du gène: SLC35B4
• Identification du gène (NCBI): 84912
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC35B4 (solute carrier family 35 member B4), also known as YEA. It is expected to be located in the endoplasmic reticulum membrane, which is ubiquitinated in placenta and brain. SLC35B4 is a glycosyltransferase, which transports nucleotide sugars from the cytoplasm where they are synthesized, to the Golgi apparatus where they are utilized in the synthesis of glycoproteins, glycolipids, and proteoglycans (PMID: 15911612). Belongs to the nucleotide-sugar transporter family. SLC35B subfamily. The molecular weight of SLC35B4 is 37 kDa.

Publications

Species	Application	Title
human,mouse	WB	mBio; Cellular SLC35B4 promotes internalization during influenza A virus entry; Authors - Guangwen Wang ; KO Validated

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