Anticorps Recombinant de lapin anti-SLC38A9

SLC38A9 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240039E7

N° de cat : 83182-5-RR

Synonymes

URLC11, Up-regulated in lung cancer 11, Solute carrier family 38 member 9, Neutral amino acid transporter 9, 240039E7

---

Galerie de données de validation

• 

  WB analysis of mouse pancreas using 83182-5-RR

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 83182-5-RR (SLC38A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of U2OS using 83182-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug Slc38a9 Recombinant Antibody (83182-5-RR, Clone:240039E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

• 

  Affinity and Kinetic Characterization of 83182-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83182-5-RR against Human SLC38A9 were performed. The affinity constant is 0.715 nM.

---

Applications testées

• Résultats positifs en WB: tissu pancréatique de souris,
• Résultats positifs en FC (Intra): cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83182-5-RR cible SLC38A9 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC38A9 Protéine recombinante Ag15607
• Nom complet: solute carrier family 38, member 9
• Masse moléculaire calculée: 561 aa, 64 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC066891
• Symbole du gène: SLC38A9
• Identification du gène (NCBI): 153129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that responds to multiple environmental cues. The lysosomal amino acid transporter SLC38A9 is a physical and functional component of this sensing complex. SLC38A9 (Solute Carrier Family 38 Member 9) was first identified as a potential amino acid transporter belonging to the slc38 family. SLC38A9 has some isoforms: 64,53,57 kDa.

{{ptg:RelatedPrimaryAntibodies}}